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The authors highlight in their conclusion that the new Python library has the potential to accelerate and expand microscopy development.

We have also added an additional supplemental figure. We have addressed all of their points and have included below a detailed reference to the changes we made accordingly. We would like to thank our reviewers for their critical reading and constructive comments. Content has not been altered except for formatting. Note: This rebuttal was posted by the corresponding author to Review Commons.

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We discuss the architecture choices of Python-Microscope that overcome the performance problems often raised against Python and demonstrate the different use cases that drove its design: its integration in user facing projects, namely in the Microscope-Cockpit project in controlling complex microscopes at high speed while using the Python programming language and as a microscope simulation tool for software development. Python-Microscope supports the distribution of devices over multiple computers while maintaining synchronisation via highly precise hardware triggers. Concrete implementations are provided for a range of specific hardware and a framework exists for further expansion. Python-Microscope offers simple to use Python-based tools, abstracting differences between physical devices by providing a defined interface for different device types. Here we report Python-Microscope, a free and open source Python library for high performance control of arbitrarily complex and scalable custom microscope systems. It is also desirable to have a solution that is scalable to complex systems and translatable between components from different manufacturers. Custom built microscopes often require control of multiple hardware devices and precise hardware coordination.

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